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新型蛋白质纤维染色剂——京尼平
http://tech.QQ.com  2009年04月24日10:54   腾讯科技    我要评论(0)

腾讯科技讯 第60届Intel ISEF英特尔国际科学与工程大奖赛将于2009年5月10日到15日在美国内华达州雷诺市举行,以下是来自中国的参赛项目介绍:

新型蛋白质纤维染色剂——京尼平

成都市第七中学 文暘天 陈礼 朱晟爽

摘要  现有染发剂大多含苯二胺等致癌物质,因此寻找新型安全的蛋白质纤维染色剂是国内外的热点。本文从栀子(Gardenia jasminoides Ellis)中制备得到京尼平,探讨了京尼平与牛血清白蛋白(BSA)溶液反应的条件、影响因素和可能的呈色机理,并借助SDS-PAGE分析了染色后蛋白质的电泳行为。结果表明:在35 0C ,pH 7.5~8.5,京尼平用量为10 %的温和条件下,白发、蚕丝等蛋白质纤维在无色的京尼平溶液中30 min可被染成蓝黑色。京尼平及其环烯醚萜类似物可使相同蛋白质纤维呈现不同的色泽,颜色一月内不褪色。染色后高分子区出现多条蛋白质带推测是由于京尼平与蛋白质发生了交联作用,这明显不同于其它染料的着色机理。因此,京尼平及其类似物很有希望成为新型蛋白质纤维染色剂。

关键词 京尼平;蛋白质纤维;交联;白发;染色

以下是英文版:

Genipin - A Novel Dye for Protein Fibers

Abstract: Most of the commercially available hair dyes contain phenylenediamines or dihydroxyhenzenes, both of which may cause cancers or other abnormal and mutational diseases. In order to find a novel and safe dye, the use of herbal medicines in the production of dyes is a new approach. In this study, genipin isolated from the fruit of Gardenia jasminoides Ellis was reacted with bovine serum albumin (BSA) and protein fibers (white hair and silk) to find the optimal dyeing conditions. SDS-PAGE was used to analyze the dyeing mechanism during this research. The reaction products were added to alkaline solutions for fourteen days to test their stability. The results showed that genipin could react with protein fibers under a mild condition ( 35 degree centigrade, pH 7.5-8.0 ), for a short time (about 30 min) and with low dosages (10% (w/w) of fiber). The UV-spectrometer scan showed that the compounds of genipin and BSA had a maximun absorption peak at 586 nm. The blue-black color reaction products were stable. The results from SDS-PAGE showed that ginipin had cross-link with protein fibers . Therefore, it was distinct from most traditional dyes (natural or synthesized). Genipin, a novel kind of potential natural reactive dyestuff, can be applied to leathers, furs, silks, and wools and is also a promising safe and environmentally friendly hair-dye.

Key Words: genipin; protein fibers; cross-linking; white hair; dye

[责任编辑:brookli]

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